By Saul L. Neidleman (ed.), Allen I. Laskin (ed.)
Meant for researchers in utilized microbiology and environmental engineers, this publication covers such issues as environmental evaluate of biotechnological techniques and microbial ameliorations of haloaromatic and haloaliphatic compounds.
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Additional info for Advances in Applied Microbiology, Vol. 35
V. , Ltd. (1961); Arima (1964); Tobey and Yousten (1976); Lulla and Subrahmanyan (1954) Qadeer et al. ,Ltd. , Ltd. (1961); Lulla and Subrahmanyan (1954) Minagawa and Hamaishi (1962) Lulla and Subrahmanyan (1954) 1:l Lulla and Subrahmanyan 1:l Lulla and Subrahmanyan (1954) Lulla and Subrahmanyan (1954) Lulla and Subrahmanyan (1954) (1954) 1:l 1:1 (1976), probably with the dual objective of providing Ca2+ and pH maintenance. F. MOISTURE LEVEL Considerable work has been done on the effect of moisture levels on the extent of bacterial a-amylase production in the SSF technique.
The methodology followed by Lulla and Subrahmanyan (1954) consisted of autolysis of bacterial bran grown for 72 hours by adding 2 ml toluene, holding it for 24 hours, at room temperature, and extraction with 50 ml water. , 1980). The ratio of bacterial bran to solvent used by some workers is too high. For example, the dried bacterial bran was contacted with 16 parts water and fp extract was separated by filtration (Minagawa and Hamaishi, 1962). The residue was subsequently washed with 14 parts water and the resulting extract was again filtered.
1980). , 1945). The extract was strained through cheesecloth for clarification by Lulla and Subrahmanyan (1954). The enzyme extract was centrifuged at 0"-4"C for 20 minutes for clarification by Ramesh and Lonsane (1987a,b, 1988). The clarity of the extract achieved by following any one of the foregoing methods was similar and the clarified extract thus obtained was found to pose no interference either in enzyme assay or in liquefaction of starch (Ramesh and Lonsane, 1988). , 1961; Minagawa and Hamaishi, 1962).