By P. D. Evans
Insect body structure is at present present process a revolution with the elevated software of molecular organic thoughts to enquire the molecular mechanisms underlying the physiological responses to insect cells. Advances in Insect body structure has instituted a dedication to the ebook of top of the range reports on molecular biology and molecular genetics in components the place they supply an elevated knowing of physiological strategies in bugs. quantity 25 includes elevated assurance at the molecular biology of insect body structure.
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Extra resources for Advances in Insect Physiology, Vol. 25
1991), downstream of the P-galactosidase coding region. The sequence of the second Bsu36I recognition site was designed to preserve the coding region of ORF 1629. This virus was designated BacPAK6 (Kitts and Possee, 1993). Digestion of the virus DNA with Bsu36I removes part of ORF 603, the polyhedrin gene promoter, the P-galactosidase coding region and part of ORF 1629. When the Bsu361digested BacPAK6 virus DNA was used to transfect insect cells, very low recoveries of infectious virus were attained.
Therefore, in some cases it may be advantageous to use a stable expression system utilizing non-infected cells. , 1990b). 1), it can be used to express foreign genes in transformed insect cell lines, thus allowing the foreign gene to be continuously synthesized. The stable and continuous expression of tissue plasminogen activator and E. , 1990b) have been described, although the levels of foreign protein synthesized were much reduced compared with those obtained via recombinant virus-infected cells.
Foreign genes are inserted directly under the p10 promoter by co-transfection of insect cells with a p10 transfer vector and purified wild-type AcMNPV DNA. Other AcMNPV gene promoters may also be used for the expression of foreign genes in virus-infected cells. Several late gene promoters, while not as active as the very late polyhedrin and p10 gene promoters, can produce useful quantities of recombinant material. One problem encountered when using the late gene promoters is that they are normally associated with genes responsible for producing virus particle structural proteins.