By Silvio Garattini
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Extra info for Advances in Pharmacology and Chemotherapy Volume 14
Katz er a f . (1972) reported a simple device for adapting Kato’s quantitative technique to field studies or situations in which an analytical balance is not available. 37-mm thick card with a 6-mm diameter hole in it. After screening the feces through a stainlesssteel sieve (150 pm), the disposable card is placed on a slide and the hole filled with the material. After covering the feces with a cellophane cover slip previously kept at least for 24 hours in a special solution (100 rnl of water, 100 ml of glycerin, and 1 ml of a 3% aqueous solution of malachite green), the slide is inverted and pressed down.
1966). , 1962), the antimonials also affect the miracidium inside mature eggs, thus interfering with its viability and infectivity (Friedheim and De Jongh, 1959; Salem and El Sherif, 1961). When treatment with antimonials was performed at the time of cercariat exposure or a few days after, these drugs exerted a schistosomistatic action, that is, they delayed the development of schistosomula into adult worms (Ercoli and Payares, 1974). Schubert (1948~)demonstrated that different trivalent antimonial compounds, including Fuadin and tartar emetic, were able to reduce the number of developing worms and to protect a significant number of mice from infection when these compounds were administered for 5 consecutive days immediately after exposure to S.
The second generation was totally resistant to hycanthone action. Yarinsky repeated such experiments using S. mansoni from the same origin and also from another source than that already used by 42 NAFTALE KATZ Rogers and Bueding (1971). He confirmed the resistance of one strain, but he also reported that, with S . mansoni maintained in the SterlingWinthrop Research Institute, resistance had not been induced (Archer and Yarinsky, 1972). Further studies demonstrated that resistance to hycanthone was maternally transferred, and remained stable for several generations.