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In the single-isotope technique, an isotope of the mineral is given by mouth. A complete faecal collection is carried out until virtually all the unabsorbed oral tracer has been recovered. The fraction of administered tracer that has been absorbed is calculated from the difference between the amount of the oral dose and the faecal isotope recovery. This technique has the benefit that the calculated absorption represents only the dietary component of the element that is absorbed and does not include endogenous secretory losses.

1965). Early in the morning of the study, subjects are instructed to empty their bladders and are given breakfast. Toward the end of the meal, the subjects are given an isotope of calcium that has been premixed (and allowed to equilibrate in the refrigerator for 12–24 h) with milk or juice. , 1999). , 1992a). The oral tracer mixes with the intravenous (IV) tracer, which serves to ‘normalize’ for variations in calcium distribution pool mass among subjects (Abrams, 1998). After administration of the tracers, a complete 24 h urine collection is carried out.

1994) Measurement of tissue protein synthesis rates in vivo: a critical analysis of contrasting methods. American Journal of Physiology 266, E287–E297. , Udall, J. R. (1980) Albumin synthesis in young and elderly subjects using a new stable isotope methodology: response to level of protein intake. Metabolism 29, 1075–1086. L. W. (1978) Regulation and significance of amino acid metabolism in skeletal muscle. Federal Proceedings 37, 2301–2307. R. N. (1991) Effect of exogenous growth hormone on whole-body and isolated-limb protein kinetics in burned patients.

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